Journal: Nature Communications
Article Title: TCF-1 and TOX regulate the memory formation of intestinal group 2 innate lymphoid cells in asthma
doi: 10.1038/s41467-024-52252-2
Figure Lengend Snippet: A WT or Rag1 – / – mice were stimulated with HDM, and anti-KLRG-1 mAb or isotype IgG were administered intraperitoneally as indicated. B The percentage and the number of CD45 + lineage – CD90.2 + NK1.1 – NKp46 – GATA3 + ST2 – KLRG1 + IL-17RB + ILC2s in lungs were analyzed on day 24. C Pulmonary IL-13 was detected by ELISA. D Airway resistance was measured under stimulation of methacholine. E Pulmonary histological changes were observed by hematoxylin-eosin staining. Magnification: ×200, scale bar = 100 μm. F Rag1 – / – mice were stimulated with HDM, and CD45 + lineage – CD90.2 + ST2 – KLRG1 + IL-17RB + ILC2s were sorted from siLP on D30. In vitro culturing cells were treated with anti-KLRG1 mAb or isotype control, and then adoptively transferred into untreated ILC2 depleted (anti-CD90.2 mAb or isotype control) Rag1 – / – mice via tail vein (5 × 10 5 cells/mouse). Recipient mice were intranasally treated (i.n.) with 25 μg HDM or 500 ng recombinant IL-25 for 3 consecutive days. G The percentage and the number of CD45 + lineage – CD90.2 + NK1.1 – NKp46 – GATA3 + ST2 – KLRG1 + IL-17RB + ILC2s in lungs. H , I IL-13 level in lungs homogenates H and airway resistance I was measured. J Pulmonary histological changes were analyzed. Magnification: ×200, scale bar = 100 μm. Results are representative of three independent experiments. Data was shown as Means ± SD. n = 6 in all panels except for n = 3 in D and I . Statistical comparison was conducted using unpaired, two-sided Welch’s t test, except in ( D ) and ( I ) using unpaired two-way ANOVA with the Bonferroni posttest to analyze dose response to methacholine. p values are shown on the graphs. Source data are provided as a Source Data file. Fig. 4F Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en .
Article Snippet: The culture was incubated with 0.75 μg/mL anti-KLRG-1 (clone 2F1, #BE0201; BioXCell) or isotype IgG (#BE0087; BioXCell) for 3 consecutive days before adoptive transfer.
Techniques: Enzyme-linked Immunosorbent Assay, Staining, In Vitro, Control, Recombinant, Comparison
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